Coding

Part:BBa_K1321371:Design

Designed by: Xenia Spencer-Milnes   Group: iGEM14_Imperial   (2014-10-17)


Linker-CBDcipA-Linker + sfGFP with LacI promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 383
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 383
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 383
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 383
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 383
    Illegal NgoMIV site found at 230
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 962


Design Notes

Linker either side of the CBD so fusions should be feasible at both N and C-termini


Source

This is cloned from the specified biobricks

References